Histochemistry of gingiva pdf

Gingiva definition of gingiva by medical dictionary. Healthy gingiva read the description and select the correct image. Having read this chapter, the reader should have a working knowledge of the various conditions that can cause generalised red, white and pigmented areas on the gingivae. The width of attached gingiva is the distance between the mucogingival junction to projection of the external surface of the bottom of the sulcus or the periodontal pocket. Read histochemical demonstration of lectinbinding sites and keratin in inflamed human gingiva, journal of periodontal research on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Assessment of the width of attached gingiva using different methods in various age groups.

View the article pdf and any associated supplements and figures for a period of 48 hours. Abstractthe distribution of a proteolytie enzyme aminopeptidase in gingival tissue has been ascertained using two different histochemical. Histochemistry of viable frozenstored human gingiva. They consist of mucosal tissue that covers the alveolar processes of the maxilla and mandible and finish at the. The soft tissue surrounding the neck of individual teeth as well as covering the alveolar bone. Orban first described the term attached gingiva as that part of the gingiva that is firmly attached to the underlying tooth and bone and is stippled on the surface. Attached gingiva continous with the marginal gingiva. Gingivitis gingivitis is an infection that is an early sign of gum disease. Introduction lipomas are common soft tissue mesenchymal neoplasms, with 1520% of cases involving the head and neck region and only 14% affecting the oral cavity. Threedimensional images showed the vascular network in the stroma of gingiva with one capillary loop in each stromal papilla invading into the epithelium. Histochemistry of the gingivaiii the distribution of.

The histochemistry of gingiva has been looked into at depth and various enzymes and biochemical pathways. Healthy attached gingiva is pink in color, with some areas of melanin pigmentation possible see figures 210 and 922. This chapter aims to identify those conditions that may produce widespread colour changes of the gingivae. We here present a case of oral lipoma on the gingiva in a 65yr old female. Gingival and dentogingival junctional tissue pocket. The purpose of this study was to analyze a case of idiopathic gingival fibromatosis, using histochemical and immunohistochemical staining and transmission electron microscopy. Gingival fibromatosis is a rare disease characterized by enlargement of the gingiva. In the case of dpp iv, enzyme localization was confirmed by immunocytochemistry with mouse. The color may also vary depending on the location of the epithelium. It includes content provided to the pmc international. A clinical and microscopic study of the free and attached gingiva. Isolation and characterization of gingival fibroblasts. Odontogenic ultrastructural and histochemical studies. A clinical study gouri bhatia 1, ashish kumar 2, manish khatri 2, mansi bansal 2, sameer saxena 2 1 department of periodontology, eklavya dental college, kotputli, jaipur, rajasthan, india 2 department of periodontology, institute of dental studies and technologies, modinagar.

Recognition of normal gingiva, gingival infection, deeper periodontal involvement depends on disciplined, stepbystep examination dictate what we need to do, reversed on healthy tissue back to healthy state, chemotherapeutic agents. Diffuse macular pigmentation of the gingiva in a patient with addisons disease. Gingival description dentistry 104 with clossen at. Enlarged gingiva a thorough examination of the gingiva includes evaluation of color, size, shape form or contour, consistency, surface texture, position of the gingival margin and junctional epithelium, mucogingival junctions, bleeding and exudate. Pages in category gingiva the following 10 pages are in this category, out of 10 total. Pdf the oxygen metabolism of gingiva remains an important, yet unexplored phenomenon. Pdf in the present study, 3d histochemistry and imaging methodology is described for human gingiva to analyze its vascular network. Histochemistry combines the methods of histology with those of chemistry or biochemistry, to reveal the biochemical composition of tissues and cells.

Quintarelli department of oral pathology, university of alabama medical centre, birmingham, alabama, u. Pigmented lesions were also present on the buccal and labial mucosa in contrast to the situation for physiologic pigmentation the marginal gingiva is involved in this case. Histochemistry ofthe muco substance indicated that hyaluronic acid andchondroitin sulphate werepresent. Firm and nodular depending on whether the dominant surface is exudative and fibrotic. Comparative histochemical and biochemical studies of mast cell tryptase in human gingiva.

In addition to adding to our understanding of physiologic processes in the gingiva this information provides the guidelines for interpreting the changes which occur in gingival diseases. Assessing the width of the attached gingiva will help in assessing the risk for a periodontium to be affected by disease measurement of the width of attached gingiva in an indian subpopulation dhalkari chandulal, et, al. Histochemical and immunocytochemical localization of. In drug induced gingival enlargement a nodular surface is seen. Histochemical demonstration of lectinbinding sites and. In the present study, 3d histochemistry and imaging methodology is described for human gingiva to analyze its vascular network. These are found in the oral cavity or mouth of a human being surrounding part of the teeth. How to fix forward head posture 3 easy exercises from a chiropractor duration.

Retractable gingiva a thorough examination of the gingiva includes evaluation of color, size, shape form or contour, consistency, surface texture, position of the gingival margin and junctional epithelium, mucogingival junctions, bleeding and exudate. The patient was a 39yearold japanese man, in whom the gingiva was enlarged throughout the. Progress in histochemistry and cytochemistry 51 2016 923 11 1. The distribution of a proteolytic enzyme aminopeptidase in gingival tissue has been ascertained using two different histochemical methods. Published monthly, jhc emphasizes research which employs in situ evaluation of biology central to the hypothesis. Scribd is the worlds largest social reading and publishing site. Department of periodontology, kings college school of medicine and dentistry, london, uk. Plaque is a layer that sticks to your teeth from food, germs and mucus. Europe pmc is an elixir core data resource learn more. Research associate and clinical instructor, department of periodontology, the royal dental college, aaarhus, denmark. Using an enzymatic digestion procedure, gingival cell suspensions containing alppositive fibroblasts were prepared without affecting alp activity. The width of the attached gingiva varies according to its location. More recently histochemical staining techniques 2 have been. March 19, 2020 the gingiva is the anatomical term for gums.

Clinical, histological and histochemical study of the. D ipeptidyl peptidase dpp ii and iv activities were demonstrated in unfixed cryostat sections of gingival tissue from chronic periodontitis patients using histochemistry with 2. Histochemistry of gingiva staining histology scribd. Makilko katsuki and sawako hojo, long term observation of gingiva enlargement technique application of wound healing materials, nihon shishubyo gakkai kaishi. Quintarelli university of alabama medical centre, birmingham, alabama, u. Europe pmc is a service of the europe pmc funders group, in partnership with the european bioinformatics institute. Pdf threedimensional histochemistry and imaging of human. They surround the teeth and provide a seal around them. Gingival structure, function, and the latest research.

Gingival colour changes generalised pocket dentistry. Fifteen human gingiva samples without signs of inflammation were. Pdf threedimensional histochemistry and imaging of. The gums often do not hurt with gingivitis, but treatment is very important. Anatomical relationship of normal gingiva in facial view a and in crosssection b. Compared with the soft tissue linings of the lips and cheeks, most of the gingivae are tightly bound to the underlying bone which helps resist the friction of food passing over them. Histochemical, immunohistochemical, and ultrastructural. The tissue is fibrous and continuous with the periodontal ligament and mucosal covering.

Clinically normal gingiva showed slight metachromasia. A solution of diazo1htetrazole, freshly prepared by the diazotization of 5amino1htetrazole under conditions to avoid explosion, was adjusted to ph 8. Histochemistry of the gingivaiii the distribution of amino. The attached gingiva is a masticatory mucosa see chapter 9. Histochemical techniques are based on precise chemical rationales for their ability to identify or stain different biochemical substances. The tissue when dried is dull, firm, and immobile, with varying amounts of stippling. Histochemistry is the study of identification and distribution of chemical compounds within and between biological cells using histological techniques such as. Abstractthe metachromatic phenomenon in the gingival connective tissue was studied. The attached gingiva is stippled the marginal gingiva is not stippled produced by rete pegs stippling is a feature of healthy gingiva the gingival surface may be. Detailed discussion of gingiva, periodontal ligament.

Good oral hygiene keeps this seal intact, while bad oral. Over time this build up can lead to infection of the gums. It occurs when plaque builds up between the teeth and gums. Gingiva are part of the soft tissue lining of the mouth. Histochemical distribution of acid phosphatase in human gingiva. The distribution of aminopeptidase in normal and inflammatory conditions. Harrison fromthe departmentoforalpathology, kings collegehospital dentalschool, london synopsis anodontogenic myxomaofa maxilla has beenexamined. The gingiva is attached to the tooth, forming a seal that protects the underlying bone and helps provide a barrier against infection.

Abstractthe distribution of a proteolytie enzyme aminopeptidase in gingival tissue has been ascertained using two different histochemical methods. Threedimensional histochemistry and imaging of human gingiva. Measurement of the width of attached gingiva in an indian. Clearing methodologies in historical perspective the german anatomist werner spalteholz 18611940 spalteholz, 1911, 1914 was the. Histochemistry on cryostat sections of gingiva samples after 3d imaging validated structures visualized in 3d.